Maria Auditore da Firenze (née de' Mozzi; 1432 – 1504) was a Florentine noblewoman of the House of Auditore and a member of the Italian Brotherhood of Assassins. Her husband was Giovanni Auditore da Firenze, a prominent Assassin in 15th century Italy, and she was the mother of Ezio Auditore da Firenze, who would become the Mentor of the Italian Brotherhood and was responsible for destroying the Templar Italian Rite's plans for Italy.
Auditore was stricken with grief after her husband, Giovanni, and sons Federico and Petruccio were hanged by Uberto Alberti on the orders of Rodrigo Borgia. She moved with the remainder of her family to Monterrigioni. The death of most of her family caused Maria to not speak for two decades, only recovering after Ezio brought her one hundred eagle feathers, with feather gathering being a pastime of Petruccio.
In 1500, Maria and the remainder of her family fled Monterrigioni during the siege led by Cesare Borgia, moving to Rome and assisting her daughter Claudia in the operation of the Rosa in Fiore brothel. She later died from a long-term illness in 1504, devastating her family.