The 32nd Ohio Infantry Regiment was an Ohian regiment of the Union Army that was active from July 1861 to 20 July 1865 during the American Civil War. The regiment was organized at Mansfield, and it first fought at the Battle of Cheat Mountain before fighting in the Valley Campaign of 1862, the Battle of Harpers Ferry, the Vicksburg campaign, the Atlanta Campaign, the March to the Sea, and the Carolinas campaign.